验证数据展示
经过测试的应用
| Positive WB detected in | U-87 MG cells, human placenta tissue, mouse cerebellum tissue, mouse lung tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27391-1-AP targets PPAP2B in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | PPAP2B fusion protein Ag26422 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphatidic acid phosphatase type 2B |
| 别名 | PAP2 beta, PAP2 b, PAP 2b, LPP3, EC:3.1.3.- |
| 计算分子量 | 35 kDa |
| 观测分子量 | 30-40 kDa |
| GenBank蛋白编号 | BC009196 |
| 基因名称 | PPAP2B |
| Gene ID (NCBI) | 8613 |
| RRID | AB_3669598 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O14495 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Phosphatidic acid phosphatase type 2B (PPAP2B), also known as Lipid phosphate phosphatase 3 (LPP3), is a member of the phosphatidic acid phosphatase (PAP) family. PPAP2B is an integral membrane protein that inactivates lysophosphatidic acid, was implicated in coronary artery disease (CAD) by genomewide association studies (PMID: 26034042). PPAP2B can be detected at least two immunoreactive bands with different mobility, ranging from 36 to 50 kDa, which may reflect oligomer formation and/or post-translational protein modifications, such as glycosylation (PMID: 28364255).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPAP2B antibody 27391-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |