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PPAPDC3 Polyclonal antibody

PPAPDC3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 20635-1-AP

Print datasheet

Synonyms

C9orf67, KIAA0515, NET39, PPAPDC3

验证数据展示

  • WB analysis of mouse skeletal muscle using 20635-1-AP

    WB analysis of mouse skeletal muscle using 20635-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20635-1-AP (PPAPDC3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse skeletal muscle using 20635-1-AP

    IP experiment of mouse skeletal muscle using 20635-1-AP

    IP result of anti-PPAPDC3 (IP:20635-1-AP, 4ug; Detection:20635-1-AP 1:800) with mouse skeletal muscle tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

The immunogen of 20635-1-AP is PPAPDC3 Fusion Protein expressed in E. coli.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen PPAPDC3 fusion protein Ag14676 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphatidic acid phosphatase type 2 domain containing 3
Synonyms C9orf67, KIAA0515, NET39, PPAPDC3
Calculated Molecular Weight 271 aa, 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC006362
Gene Symbol PPAPDC3
Gene ID (NCBI) 84814
RRIDAB_10696180
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NBV4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPAPDC3(Phosphatidic acid phosphatase type 2 domain-containing protein 3) is also named as C9orf67 and belongs to the PA-phosphatase related phosphoesterase family. It plays a role as negative regulator of myoblast differentiation, in part through effects on MTOR signaling.

实验方案

Product Specific Protocols
WB protocol for PPAPDC3 antibody 20635-1-APDownload protocol
IP protocol for PPAPDC3 antibody 20635-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Genome Biol

Specific nuclear envelope transmembrane proteins can promote the location of chromosomes to and from the nuclear periphery.

Authors - Zuleger Nikolaj N
View Article
humanWB

Cells

Lamin A/C Assembly Defects in LMNA-Congenital Muscular Dystrophy Is Responsible for the Increased Severity of the Disease Compared with Emery-Dreifuss Muscular Dystrophy.

Authors - Anne T Bertrand
View Article
humanWB

Front Cell Dev Biol

Nuclear envelope transmembrane proteins involved in genome organization are misregulated in myotonic dystrophy type 1 muscle

Authors - Vanessa Todorow
View Article