PPAR Gamma Recombinant antibody
PPAR Gamma Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
230374A3
验证数据展示
经过测试的应用
Positive WB detected in | MCF-7 cells, HepG2 cells, L02 cells, C6 cells |
Positive IHC detected in | human placenta tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | HeLa cells, K562 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 81490-5-RR is PPAR Gamma Fusion Protein expressed in E. coli.
Tested Applications | WB, IHC, FC (Intra), ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | PPAR Gamma fusion protein Ag10005 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | peroxisome proliferator-activated receptor gamma |
Synonyms | PPARG, PPARγ, PPAR-gamma, Peroxisome proliferator-activated receptor gamma, NR1C3 |
Calculated Molecular Weight | 58 kDa |
Observed Molecular Weight | 50-60 kDa |
GenBank Accession Number | BC006811 |
Gene Symbol | PPARG |
Gene ID (NCBI) | 5468 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P37231 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for PPAR Gamma antibody 81490-5-RR | Download protocol |
IHC protocol for PPAR Gamma antibody 81490-5-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |