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PPARA Polyclonal antibody

PPARA Polyclonal Antibody for WB, IP, ELISA
Cat No. 15540-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (5)

应用

WB, IP, ELISA and More (3)

hPPAR, NR1C1, PPAR, PPAR alpha, PPARA, PPARα

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inC2C12 cells
Positive IP detected inU-937 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15540-1-AP targets PPARA in WB, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IF, CoIP, ChIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig, chicken, bovine, hamster, goat
免疫原 PPARA fusion protein Ag7896 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peroxisome proliferator-activated receptor alpha
别名 hPPAR, NR1C1, PPAR, PPAR alpha, PPARA, PPARα
计算分子量 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC000052
基因名称 PPARA
Gene ID (NCBI) 5465
RRIDAB_2252506
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ07869
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Peroxisome proliferator-activated receptor alpha (PPARA) is a ligand-activated transcription factor that belongs to the PPAR nuclear receptor superfamily. PPARA is essential in the modulation of lipid transport and metabolism, mainly through activating mitochondrial and peroxisomal fatty acid β-oxidation pathways. In addition, PPARA seems to decrease inflammation mainly through direct interaction with NF-κB, causing inhibition of its signaling pathway or reducing the activated levels of NF-κB and subsequent inflammation. Furthermore, PPARA was implicated in the attenuation of oxidative stress in alcoholic liver disease when treated with polyenephosphatidylcholine through downregulation of ROS-generating enzymes such as ethanol-inducible cytochrome P450 2E1 (CYP2E1), acyl-CoA oxidase, and NADPH oxidase. PPARA exists two isoforms and molecular weight of PPARA isoforms are 52 kDa and 22 kDa. The ability of a retinoid X receptor (RXR) to heterodimerize with many nuclear receptors, including LXR, PPAR, NGF1B and RAR, underscores its pivotal role within the nuclear receptor superfamily. Among these heterodimers, PPAR:RXR is considered an important signalling mediator of both PPAR ligands, such as fatty acids, and 9-cis retinoic acid (9-cis RA), an RXR ligand. (PMID: 15103326 ). PPARA can form Heterodimer with RXRA and molecular weight of Heterodimer is about 110 kDa.

实验方案

Product Specific Protocols
WB protocol for PPARA antibody 15540-1-APDownload protocol
IP protocol for PPARA antibody 15540-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nat Commun

Hyodeoxycholic acid ameliorates nonalcoholic fatty liver disease by inhibiting RAN-mediated PPARα nucleus-cytoplasm shuttling

Authors - Jing Zhong
humanWB

Sci Adv

Small-molecule inhibitor targeting orphan nuclear receptor COUP-TFII for prostate cancer treatment.

Authors - Leiming Wang
mouseWB

Hepatology

The histone methyltransferase Suv39h2 contributes to nonalcoholic steatohepatitis in mice.

Authors - Zhiwen Fan
mouseWB

Nat Commun

Deficiency of intestinal Bmal1 prevents obesity induced by high-fat feeding.

Authors - Fangjun Yu
humanWB

Nat Commun

N1-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.

Authors - Yanying Wang
mouseWB

Nat Commun

Hepatic HuR modulates lipid homeostasis in response to high-fat diet.

Authors - Zhuojun Zhang
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