- Featured Product
- KD/KO Validated
PPAR Gamma Polyclonal antibody
PPAR Gamma Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, human brain tissue, human heart tissue, mouse ovary tissue, mouse heart tissue, mouse testis tissue, mouse liver tissue, MCF-7 cells, 3T3-L1 cells, rat brain tissue, HL-60 cells, mouse brain tissue, rat liver tissue |
Positive IHC detected in | human prostate cancer tissue, human colon cancer tissue, rat colon tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 8 publications below |
IHC | See 2 publications below |
产品信息
22061-1-AP targets PPAR Gamma in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | PPAR Gamma fusion protein Ag17136 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | peroxisome proliferator-activated receptor gamma |
Synonyms | CIMT1, NR1C3, PPAR gamma, PPARG, PPARG2, PPARγ |
Calculated Molecular Weight | 505 aa, 58 kDa |
Observed Molecular Weight | 66-70 kDa |
GenBank Accession Number | BC006811 |
Gene Symbol | PPARG |
Gene ID (NCBI) | 5468 |
RRID | AB_11182268 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P37231 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for PPAR Gamma antibody 22061-1-AP | Download protocol |
IHC protocol for PPAR Gamma antibody 22061-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Genes Dev Loss of TLE3 promotes the mitochondrial program in beige adipocytes and improves glucose metabolism. | ||
Free Radic Biol Med Hydrogen sulfide alleviates particulate matter-induced emphysema and airway inflammation by suppressing ferroptosis. | ||
Biochem Pharmacol A novel pyrazole-containing indolizine derivative suppresses NF-κB activation and protects against TNBS-induced colitis via a PPAR-γ-dependent pathway. | ||
Front Pharmacol The Active Components of Fuzheng Huayu Formula and Their Potential Mechanism of Action in Inhibiting the Hepatic Stellate Cells Viability - A Network Pharmacology and Transcriptomics Approach. | ||
Front Pharmacol Alpha-Asaronol Alleviates Dysmyelination by Enhancing Glutamate Transport Through the Activation of PPARγ-GLT-1 Signaling in Hypoxia-Ischemia Neonatal Rats. |