验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, human liver tissue, human plasma tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
13313-1-AP targets PPBP,NAP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Immunogen | PPBP,NAP2 fusion protein Ag4147 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | pro-platelet basic protein (chemokine (C-X-C motif) ligand 7) |
| Synonyms | B TG1, Beta TG, C X C motif chemokine 7, CTAP III, CTAP3, CTAPIII, CXCL7, LA PF4, LDGF, MDGF, NAP 2, NAP 2(1 63), NAP-2, PBP, Platelet basic protein, PPBP, SCYB7, Small inducible cytokine B7, TC1, TC2, TGB, TGB1, THBGB, THBGB1 |
| Calculated Molecular Weight | 128 aa, 14 kDa |
| Observed Molecular Weight | 8-14 kDa |
| GenBank Accession Number | BC028217 |
| Gene Symbol | PPBP |
| Gene ID (NCBI) | 5473 |
| RRID | AB_10597238 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P02775 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PPBP, slso named as NAP2, or β-Thromboglobulin, is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPBP,NAP2 antibody 13313-1-AP | Download protocol |
| IHC protocol for PPBP,NAP2 antibody 13313-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
