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PPBP,NAP2 Polyclonal antibody

PPBP,NAP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13313-1-AP

Print datasheet

Synonyms

B TG1, Beta TG, C X C motif chemokine 7, CTAP III, CTAP3, CTAPIII, CXCL7, LA PF4, LDGF, MDGF, NAP 2, NAP 2(1 63), NAP-2, PBP, Platelet basic protein, PPBP, SCYB7, Small inducible cytokine B7, TC1, TC2, TGB, TGB1, THBGB, THBGB1

验证数据展示

  • WB analysis of mouse liver using 13313-1-AP

    WB analysis of mouse liver using 13313-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 13313-1-AP (PPBP,NAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 13313-1-AP

    IHC staining of human placenta using 13313-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13313-1-AP (PPBP,NAP2 antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human placenta using 13313-1-AP

    IHC staining of human placenta using 13313-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13313-1-AP (PPBP,NAP2 antibody at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse liver tissue, human liver tissue, human plasma tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13313-1-AP targets PPBP,NAP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen PPBP,NAP2 fusion protein Ag4147 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pro-platelet basic protein (chemokine (C-X-C motif) ligand 7)
Synonyms B TG1, Beta TG, C X C motif chemokine 7, CTAP III, CTAP3, CTAPIII, CXCL7, LA PF4, LDGF, MDGF, NAP 2, NAP 2(1 63), NAP-2, PBP, Platelet basic protein, PPBP, SCYB7, Small inducible cytokine B7, TC1, TC2, TGB, TGB1, THBGB, THBGB1
Calculated Molecular Weight 128 aa, 14 kDa
Observed Molecular Weight 8-14 kDa
GenBank Accession NumberBC028217
Gene Symbol PPBP
Gene ID (NCBI) 5473
RRIDAB_10597238
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02775
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPBP, slso named as NAP2, or β-Thromboglobulin, is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.

实验方案

Product Specific Protocols
WB protocol for PPBP,NAP2 antibody 13313-1-APDownload protocol
IHC protocol for PPBP,NAP2 antibody 13313-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

Mutant Huntingtin stalls ribosomes and represses protein synthesis in a cellular model of Huntington disease.

Authors - Mehdi Eshraghi
View Article