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PPCDC Polyclonal antibody

PPCDC Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16270-1-AP

Print datasheet

Synonyms

CoaC, MDS018, PPC DC, PPCDC, UNQ9365/PRO34154

验证数据展示

  • WB analysis using 16270-1-AP

    WB analysis using 16270-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16270-1-AP (PPCDC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, human placenta tissue, HL-60 cells, K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16270-1-AP targets PPCDC in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen PPCDC fusion protein Ag9337 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphopantothenoylcysteine decarboxylase
Synonyms CoaC, MDS018, PPC DC, PPCDC, UNQ9365/PRO34154
Calculated Molecular Weight 204 aa, 22 kDa
Observed Molecular Weight22-25 kDa, 14 kDa
GenBank Accession NumberBC014409
Gene Symbol PPCDC
Gene ID (NCBI) 60490
RRIDAB_2299841
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96CD2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCDC, one of the last enzymes in this pathway, converts phosphopantothenoylcysteine to 4-prime-phosphopantetheine. The antibody was generated against full length human recombinant PPCDC, which is a bright yellow protein, indicating the presence of flavins. The antibody solution gives yellow color, too. It may be caused by elution of flavins during affinity purification with PPCDC-protein column.

实验方案

Product Specific Protocols
WB protocol for PPCDC antibody 16270-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols