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PPCS Polyclonal antibody

PPCS Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 18001-1-AP

Print datasheet

Synonyms

COAB, PPC synthetase, PPCS

验证数据展示

  • WB analysis of HeLa using 18001-1-AP

    WB analysis of HeLa using 18001-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18001-1-AP (PPCS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 18001-1-AP

    IHC staining of human kidney using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 18001-1-AP

    IHC staining of human kidney using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

18001-1-AP targets PPCS in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen PPCS fusion protein Ag12491 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphopantothenoylcysteine synthetase
Synonyms COAB, PPC synthetase, PPCS
Calculated Molecular Weight 311 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC106064
Gene Symbol PPCS
Gene ID (NCBI) 79717
RRIDAB_10548725
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HAB8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPCS(Phosphopantothenate--cysteine ligase) is also named as COAB and belongs to the PPC synthetase family. It catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. It can be exsit as a homodimer(PMID:12906824).

实验方案

Product Specific Protocols
WB protocol for PPCS antibody 18001-1-APDownload protocol
IHC protocol for PPCS antibody 18001-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Int J Mol Sci

Regulation of the CoA Biosynthetic Complex Assembly in Mammalian Cells.

Authors - Jovana Baković
View Article