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验证数据展示

  • WB analysis using 12875-1-AP

    WB analysis using 12875-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12875-1-AP (PPEF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 12875-1-AP

    IHC staining of mouse testis using 12875-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12875-1-AP (PPEF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 12875-1-AP

    IHC staining of mouse testis using 12875-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12875-1-AP (PPEF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 12875-1-AP

    WB analysis of HeLa using 12875-1-AP

    WB result of PPEF1 antibody (12875-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPEF1 transfected HeLa cells.

  • Featured Product
  • KD/KO验证

PPEF1 Polyclonal antibody

PPEF1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12875-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

PP7, PPEF, PPEF 1, PPEF1, PPP7C

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, PC-3 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12875-1-AP targets PPEF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 PPEF1 fusion protein Ag3553 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase, EF-hand calcium binding domain 1
别名 PP7, PPEF, PPEF 1, PPEF1, PPP7C
计算分子量 653 aa, 76 kDa
观测分子量 69-76 kDa
GenBank蛋白编号BC036026
基因名称 PPEF1
Gene ID (NCBI) 5475
RRIDAB_2166375
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14829
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPEF1 antibody 12875-1-APDownload protocol
IHC protocol for PPEF1 antibody 12875-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols