验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14175-1-AP targets Liprin Alpha 1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, monkey |
| 免疫原 | Liprin Alpha 1 fusion protein Ag5381 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1 |
| 别名 | PPFIA1, LIP-1, LIP1, LIP.1, LIP 1 |
| 计算分子量 | 1202 aa, 136 kDa |
| 观测分子量 | 140-160 kDa |
| GenBank蛋白编号 | BC034046 |
| 基因名称 | Liprin Alpha 1 |
| Gene ID (NCBI) | 8500 |
| RRID | AB_2171592 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13136 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Liprin alpha 1, encoded by the PPFIA1 gene, is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. Liprin alpha 1 may regulate the disassembly of focal adhesions and thus help orchestrate cell-matrix interactions (PMID: 7796809). Alternatively spliced transcript variants encoding distinct isoforms have been described.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Liprin Alpha 1 antibody 14175-1-AP | Download protocol |
| IHC protocol for Liprin Alpha 1 antibody 14175-1-AP | Download protocol |
| IF protocol for Liprin Alpha 1 antibody 14175-1-AP | Download protocol |
| IP protocol for Liprin Alpha 1 antibody 14175-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Structural basis of liprin-α-promoted LAR-RPTP clustering for modulation of phosphatase activity. | ||
EMBO J The CEP170B-KIF2A complex destabilizes microtubule minus ends to generate polarized microtubule network
| ||
Nat Commun PPFIA1 drives active α5β1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis.
| ||
Elife Local activation of focal adhesion kinase orchestrates the positioning of presynaptic scaffold proteins and Ca2+ signalling to control glucose-dependent insulin secretion. | ||
Mol Oncol Liprin-α1 contributes to oncogenic MAPK signaling by counteracting ERK activity
| ||
Stem Cells Transl Med Enhanced structure and function of human pluripotent stem cell-derived beta-cells cultured on extracellular matrix. |
