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验证数据展示

  • WB analysis of HepG2 using 13961-1-AP

    WB analysis of HepG2 using 13961-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13961-1-AP (PPFIBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 13961-1-AP

    IF Staining of U2OS using 13961-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PPFIBP1 antibody (13961-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HepG2 using 13961-1-AP

    FC experiment of HepG2 using 13961-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PPFIBP1 Polyclonal antibody (13961-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IP experiment of HepG2 using 13961-1-AP

    IP experiment of HepG2 using 13961-1-AP

    IP result of anti-PPFIBP1 (IP:13961-1-AP, 4ug; Detection:13961-1-AP 1:500) with HepG2 cells lysate 2400ug.

PPFIBP1 Polyclonal antibody

PPFIBP1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Cat No. 13961-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), IP, ELISA and More (1)

别名

PTPRF-interacting protein-binding protein 1, Protein tyrosine phosphatase receptor type f polypeptide-interacting protein-binding protein 1, Liprin-beta-1, KIAA1230, hSGT2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, DU 145 cells
Positive IP detected inHepG2 cells
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

13961-1-AP targets PPFIBP1 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用IF
经测试反应性 human
文献引用反应性human
免疫原 PPFIBP1 fusion protein Ag4884 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PTPRF interacting protein, binding protein 1 (liprin beta 1)
别名 PTPRF-interacting protein-binding protein 1, Protein tyrosine phosphatase receptor type f polypeptide-interacting protein-binding protein 1, Liprin-beta-1, KIAA1230, hSGT2
计算分子量 1011 aa, 114 kDa
观测分子量 114 kDa
GenBank蛋白编号BC050281
基因名称 PPFIBP1
Gene ID (NCBI) 8496
RRIDAB_2171631
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86W92
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPFIBP1 antibody 13961-1-APDownload protocol
IF protocol for PPFIBP1 antibody 13961-1-APDownload protocol
IP protocol for PPFIBP1 antibody 13961-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Open Biol

The structural basis of the talin-KANK1 interaction that coordinates the actin and microtubule cytoskeletons at focal adhesions

Authors - Xingchen Li
View Article
human

J Inflamm Res

Development and Validation of Diagnostic Models for Transcriptomic Signature Genes for Multiple Tissues in Osteoarthritis

Authors - Qichang Gao
View Article