验证数据展示

WB analysis of HeLa using 11607-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11607-1-AP (Cyclophilin B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human lymphoma using 11607-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PPIB antibody included
IHC staining of human lymphoma using 11607-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PPIB antibody included
IF Staining of HeLa using 11607-1-AP
Immunofluorescent analysis of HeLa cells using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
WB analysis of HeLa using 11607-1-AP
WB result of Cyclophilin B antibody (11607-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclophilin B transfected HeLa cells.
IHC staining of human normal colon using 11607-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PPIB antibody included
IHC staining of human normal colon using 11607-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PPIB antibody included

Featured Product
KD/KO验证

Cyclophilin B Polyclonal antibody

Cyclophilin B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11607-1-AP

产品说明书

引用文献(16)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

PPIB, PPIase B, Peptidyl-prolyl cis-trans isomerase B, EC:5.2.1.8, CYP-S1

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (16)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HeLa cells, U-937 cells, Raji cells, HepG2 cells, mouse liver tissue, NIH/3T3 cells, Jurkat cells, RAW 264.7 cells, rat liver tissue
Positive IHC detected in	human normal colon, human liver cancer tissue, human lymphoma tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy PPIB Ready-To-Use IHC Kit. PPIB primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:300-1:1200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 1 publications below
WB	See 14 publications below
IHC	See 3 publications below
IF	See 2 publications below

产品信息

11607-1-AP targets Cyclophilin B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, monkey
免疫原	Cyclophilin B fusion protein Ag2161 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	peptidylprolyl isomerase B (cyclophilin B)
别名	PPIB, PPIase B, Peptidyl-prolyl cis-trans isomerase B, EC:5.2.1.8, CYP-S1
计算分子量	216 aa, 24 kDa
观测分子量	19-24 kDa
GenBank蛋白编号	BC032138
基因名称	Cyclophilin B
Gene ID (NCBI)	5479
RRID	AB_2237519
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P23284
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

PPIB, also named as CYPB, SCYLP, PPIase B and CYP-S1, belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. The full-length of PPIB has 216aa (24 kDa), and a 33aa-signal peptide could be removed in the matrue form (about 19 kDa).

实验方案

Product Specific Protocols
WB protocol for Cyclophilin B antibody 11607-1-AP	Download protocol
IHC protocol for Cyclophilin B antibody 11607-1-AP	Download protocol
IF protocol for Cyclophilin B antibody 11607-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Mol Cell Metabolic Diversity in Human Non-Small Cell Lung Cancer Cells. Authors - Pei-Hsuan Chen View Article
human	WB,IHC	Am J Hum Genet PPIB mutations cause severe osteogenesis imperfecta. Authors - van Dijk Fleur S FS View Article
mouse	WB	Cell Prolif OGG1 aggravates renal ischemia-reperfusion injury by repressing PINK1-mediated mitophagy Authors - Fan Zhao View Article
human	WB,IF	Hum Mol Genet Mutations in PPIB (cyclophilin B) delay type I procollagen chain association and result in perinatal lethal to moderate osteogenesis imperfecta phenotypes. Authors - Pyott Shawna M SM View Article
mouse	WB	Cells HDAC6 Inhibition Alleviates Ischemia- and Cisplatin-Induced Acute Kidney Injury by Promoting Autophagy Authors - Lang Shi View Article
human,mouse	WB,IF	J Virol CD147/EMMPRIN acts as a functional entry receptor for measles virus on epithelial cells. Authors - Watanabe Akira A View Article

验证数据展示

WB analysis of HeLa using 11607-1-AP

IHC staining of human lymphoma using 11607-1-AP

IHC staining of human lymphoma using 11607-1-AP

IF Staining of HeLa using 11607-1-AP

WB analysis of HeLa using 11607-1-AP

IHC staining of human normal colon using 11607-1-AP

IHC staining of human normal colon using 11607-1-AP

Cyclophilin B Polyclonal antibody

Cyclophilin B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Metabolic Diversity in Human Non-Small Cell Lung Cancer Cells.

PPIB mutations cause severe osteogenesis imperfecta.

OGG1 aggravates renal ischemia-reperfusion injury by repressing PINK1-mediated mitophagy

Mutations in PPIB (cyclophilin B) delay type I procollagen chain association and result in perinatal lethal to moderate osteogenesis imperfecta phenotypes.

HDAC6 Inhibition Alleviates Ischemia- and Cisplatin-Induced Acute Kidney Injury by Promoting Autophagy

CD147/EMMPRIN acts as a functional entry receptor for measles virus on epithelial cells.