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PPID Recombinant antibody, PBS Only (Capture)

PPID Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241361B2

Cat no : 84115-1-PBS

Print datasheet

Synonyms

Cyclophilin-related protein, Cyclophilin-40, Cyclophilin D, Cyclophilin 40, 40 kDa peptidyl-prolyl cis-trans isomerase

验证数据展示

  • WB analysis using 84115-1-RR (same clone as 84115-1-PBS)

    WB analysis using 84115-1-RR (same clone as 84115-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84115-1-RR (PPID antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84115-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 84115-1-RR (same clone as 84115-1-PBS)

    FC experiment of A431 using 84115-1-RR (same clone as 84115-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug PPID Recombinant Antibody (84115-1-RR, Clone:241361B2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84115-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01018-1

    Cytometric bead array standard curve of MP01018-1

    Cytometric bead array standard curve of MP01018-1, PPID Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84115-1-PBS. Detection antibody: 84115-5-PBS. Standard: Ag3409. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP01018-2

    Cytometric bead array standard curve of MP01018-2

    Cytometric bead array standard curve of MP01018-2, PPID Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84115-1-PBS. Detection antibody: 84115-2-PBS. Standard: Ag3409. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84115-1-PBS

    Affinity and Kinetic Characterization of 84115-1-PBS

    Biolayer interferometry (BLl) kinetic assay of 84115-1-PBS against Human PPID was performed. The affinity constant is below 1 pM.

  • Affinity and Kinetic Characterization of 84115-1-RR

    Affinity and Kinetic Characterization of 84115-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84115-1-RR against Human PPID were performed. The affinity constant is 0.313 nM.

Planning an IHC experiment? We recommend our IHCeasy PPID Ready-To-Use IHC Kit. PPID primary antibody included.

产品信息

84115-1-PBS targets PPID as part of a matched antibody pair:

MP01018-1: 84115-1-PBS capture and 84115-5-PBS detection (validated in Cytometric bead array)

MP01018-2: 84115-1-PBS capture and 84115-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen PPID fusion protein Ag3409 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name peptidylprolyl isomerase D
Synonyms Cyclophilin-related protein, Cyclophilin-40, Cyclophilin D, Cyclophilin 40, 40 kDa peptidyl-prolyl cis-trans isomerase
Calculated Molecular Weight 370 aa, 41 kDa
Observed Molecular Weight41 kDa
GenBank Accession NumberBC030707
Gene Symbol PPID
Gene ID (NCBI) 5481
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08752
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PPID(Peptidyl-prolyl cis-trans isomerase D) is also named CYP40, CYPD, and belongs to the cyclophilin-type PPIase family, which accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The inactivation of the Ppid gene rescued the disease phenotype of Col6a1 deficiency (PMID:19293339). This PPID antibody detected a strong band at an apparent molecular mass of 41 kDa and a weak band at 70 kDa due to crosslink (PMID: 33320090, 29097228).