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PPID Recombinant antibody, PBS Only

PPID Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241361E4

Cat no : 84115-6-PBS

Print datasheet

Synonyms

Cyclophilin-related protein, Cyclophilin-40, Cyclophilin D, Cyclophilin 40, 40 kDa peptidyl-prolyl cis-trans isomerase

验证数据展示

  • WB analysis using 84115-6-RR (same clone as 84115-6-PBS)

    WB analysis using 84115-6-RR (same clone as 84115-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84115-6-RR (PPID antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84115-6-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 84115-6-RR (same clone as 84115-6-PBS)

    FC experiment of HepG2 using 84115-6-RR (same clone as 84115-6-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PPID Recombinant antibody (84115-6-RR, Clone:241361E4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84115-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84115-6-RR

    Affinity and Kinetic Characterization of 84115-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84115-6-RR against Human PPID were performed. The affinity constant is 0.497 nM.

Planning an IHC experiment? We recommend our IHCeasy PPID Ready-To-Use IHC Kit. PPID primary antibody included.

产品信息

The immunogen of 84115-6-PBS is PPID Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PPID fusion protein Ag3409 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name peptidylprolyl isomerase D
Synonyms Cyclophilin-related protein, Cyclophilin-40, Cyclophilin D, Cyclophilin 40, 40 kDa peptidyl-prolyl cis-trans isomerase
Calculated Molecular Weight 370 aa, 41 kDa
Observed Molecular Weight41 kDa
GenBank Accession NumberBC030707
Gene Symbol PPID
Gene ID (NCBI) 5481
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ08752
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PPID(Peptidyl-prolyl cis-trans isomerase D) is also named CYP40, CYPD, and belongs to the cyclophilin-type PPIase family, which accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The inactivation of the Ppid gene rescued the disease phenotype of Col6a1 deficiency (PMID:19293339). This PPID antibody detected a strong band at an apparent molecular mass of 41 kDa and a weak band at 70 kDa due to crosslink (PMID: 33320090, 29097228).