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PPIF-Specific Polyclonal antibody, PBS Only

PPIF-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18466-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 18466-1-AP (same clone as 18466-1-PBS)

    WB analysis using 18466-1-AP (same clone as 18466-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18466-1-AP (PPIF-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 18466-1-AP (same clone as 18466-1-PBS)

    IF Staining of MCF-7 using 18466-1-AP (same clone as 18466-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using PPIF antibody 18466-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse heart using 18466-1-AP (same clone as 18466-1-PBS)

    IP experiment of mouse heart using 18466-1-AP (same clone as 18466-1-PBS)

    IP result of anti-PPIF-Specific (IP:18466-1-AP, 3ug; Detection:18466-1-AP 1:1000) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 18466-1-AP (same clone as 18466-1-PBS)

    IHC staining of human heart using 18466-1-AP (same clone as 18466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18466-1-AP (CYPA Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PPIF antibody included
  • IHC staining of human heart using 18466-1-AP (same clone as 18466-1-PBS)

    IHC staining of human heart using 18466-1-AP (same clone as 18466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18466-1-AP (CYPA Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PPIF antibody included
Planning an IHC experiment? We recommend our IHCeasy PPIF Ready-To-Use IHC Kit. PPIF primary antibody included.

产品信息

18466-1-PBS targets PPIF-Specific in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PPIF-Specific fusion protein Ag13151 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peptidylprolyl isomerase F
Synonyms
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC005020
Gene Symbol PPIF
Gene ID (NCBI) 10105
RRIDAB_2169273
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30405
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PPIF, also named as CYP3, PPIase F and Rotamase F, belongs to the cyclophilin-type PPIase family. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIF is a mitochondrial matrix protein involved in the induction of necrotic and apoptotic cell death through the activation of the mitochondrial permeability transition (mPT) pore. This antibody is immunized with full length gene PPIF and absorbed by PPIA. So this antibody is specific to PPIF