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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 18466-1-AP (same clone as 18466-1-PBS)

    WB analysis using 18466-1-AP (same clone as 18466-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18466-1-AP (PPIF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 18466-1-AP (same clone as 18466-1-PBS)

    IF Staining of MCF-7 using 18466-1-AP (same clone as 18466-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using PPIF antibody 18466-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse heart using 18466-1-AP (same clone as 18466-1-PBS)

    IP experiment of mouse heart using 18466-1-AP (same clone as 18466-1-PBS)

    IP result of anti-PPIF (IP:18466-1-AP, 3ug; Detection:18466-1-AP 1:1000) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 18466-1-AP (same clone as 18466-1-PBS)

    IHC staining of human heart using 18466-1-AP (same clone as 18466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18466-1-AP (PPIF Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PPIF antibody included
  • IHC staining of human heart using 18466-1-AP (same clone as 18466-1-PBS)

    IHC staining of human heart using 18466-1-AP (same clone as 18466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18466-1-AP (CYPA Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PPIF antibody included

PPIF Polyclonal antibody, PBS Only

PPIF Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 18466-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

EC:5.2.1.8, CyP-M, CyP-D, Cyp D, Cyclophilin F

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PPIF Ready-To-Use IHC Kit. PPIF primary antibody included.

产品信息

18466-1-PBS targets PPIF in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PPIF fusion protein Ag13151 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peptidylprolyl isomerase F
别名 EC:5.2.1.8, CyP-M, CyP-D, Cyp D, Cyclophilin F
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC005020
基因名称 PPIF
Gene ID (NCBI) 10105
RRIDAB_2169273
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP30405
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PPIF, also named as CYP3, PPIase F and Rotamase F, belongs to the cyclophilin-type PPIase family. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIF is a mitochondrial matrix protein involved in the induction of necrotic and apoptotic cell death through the activation of the mitochondrial permeability transition (mPT) pore. This antibody is immunized with full length gene PPIF and absorbed by PPIA. So this antibody is specific to PPIF.