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PPIG Polyclonal antibody

PPIG Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 12985-1-AP

Print datasheet

Synonyms

Cyclophilin G, Clk-associating RS-cyclophilin, CASP10, CARS-Cyp, CARS-cyclophilin

验证数据展示

  • WB analysis of human liver using 12985-1-AP

    WB analysis of human liver using 12985-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12985-1-AP (PPIG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 12985-1-AP

    IHC staining of human prostate cancer using 12985-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12985-1-AP (PPIG antibody) at dilution of 1:100 (under 10x lens).

  • IF Staining of HeLa using 12985-1-AP

    IF Staining of HeLa using 12985-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12985-1-AP (PPIG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman liver tissue, Jurkat cells, NIH/3T3 cells, SH-SY5Y cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

12985-1-AP targets PPIG in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen PPIG fusion protein Ag3622 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peptidylprolyl isomerase G (cyclophilin G)
Synonyms Cyclophilin G, Clk-associating RS-cyclophilin, CASP10, CARS-Cyp, CARS-cyclophilin
Calculated Molecular Weight 739 aa, 87 kDa
Observed Molecular Weight 89 kDa
GenBank Accession NumberBC001555
Gene Symbol PPIG
Gene ID (NCBI) 9360
RRIDAB_2169281
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13427
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPIG antibody 12985-1-APDownload protocol
IHC protocol for PPIG antibody 12985-1-APDownload protocol
IF protocol for PPIG antibody 12985-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Sci Adv

High-throughput tagging of endogenous loci for rapid characterization of protein function

Authors - Joonwon Kim
View Article