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PPIL2 Polyclonal antibody

PPIL2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 12590-2-AP

Print datasheet

Synonyms

CYC4, Cyclophilin 60, Cyp 60, CYP60, hCyP 60, PPIase, PPIL2, Rotamase PPIL2

验证数据展示

  • IP experiment of HEK-293 using 12590-2-AP

    IP experiment of HEK-293 using 12590-2-AP

    IP result of anti-PPIL2 (IP:12590-2-AP, 4ug; Detection:12590-2-AP 1:300) with HEK-293 cells lysate 2600 ug.

  • WB analysis of human kidney using 12590-2-AP

    WB analysis of human kidney using 12590-2-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12590-2-AP (PPIL2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman kidney tissue, HEK-293 cells, mouse kidney tissue
Positive IP detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12590-2-AP targets PPIL2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen PPIL2 fusion protein Ag3296 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peptidylprolyl isomerase (cyclophilin)-like 2
Synonyms CYC4, Cyclophilin 60, Cyp 60, CYP60, hCyP 60, PPIase, PPIL2, Rotamase PPIL2
Calculated Molecular Weight 520 aa, 59 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC028385
Gene Symbol PPIL2
Gene ID (NCBI) 23759
RRIDAB_2169606
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13356
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPIL2 antibody 12590-2-APDownload protocol
IP protocol for PPIL2 antibody 12590-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Front Cardiovasc Med

Pilose Antler Peptide-3.2KD Ameliorates Adriamycin-Induced Myocardial Injury Through TGF-β/SMAD Signaling Pathway.

Authors - Yan Xu
View Article