验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HCT 116 cells, mouse kidney tissue, HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
12538-1-AP targets PPIL4 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | PPIL4 fusion protein Ag3249 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | peptidylprolyl isomerase (cyclophilin)-like 4 |
| 别名 | PPIase, Peptidyl-prolyl cis-trans isomerase-like 4, HDCME13P, EC:5.2.1.8, Cyclophilin-like protein PPIL4 |
| 计算分子量 | 492 aa, 57 kDa |
| 观测分子量 | 58-65 kDa |
| GenBank蛋白编号 | BC016984 |
| 基因名称 | PPIL4 |
| Gene ID (NCBI) | 85313 |
| RRID | AB_10644441 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WUA2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPIL4 antibody 12538-1-AP | Download protocol |
| IF protocol for PPIL4 antibody 12538-1-AP | Download protocol |
| IP protocol for PPIL4 antibody 12538-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
