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验证数据展示

  • WB analysis of HeLa using 17452-1-AP

    WB analysis of HeLa using 17452-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17452-1-AP (PPIL6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 17452-1-AP

    IF Staining of HeLa using 17452-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17452-1-AP (PPIL6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

PPIL6 Polyclonal antibody

PPIL6 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17452-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Rotamase PPIL6, Probable inactive peptidyl-prolyl cis-trans isomerase-like 6, PPIase, Cyclophilin-like protein PPIL6, Cyclophilin like protein PPIL6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

17452-1-AP targets PPIL6 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 PPIL6 fusion protein Ag11578 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peptidylprolyl isomerase (cyclophilin)-like 6
别名 Rotamase PPIL6, Probable inactive peptidyl-prolyl cis-trans isomerase-like 6, PPIase, Cyclophilin-like protein PPIL6, Cyclophilin like protein PPIL6
计算分子量 311 aa, 35 kDa
观测分子量 30 kDa
GenBank蛋白编号BC038716
基因名称 PPIL6
Gene ID (NCBI) 285755
RRIDAB_2237569
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IXY8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPIL6 antibody 17452-1-APDownload protocol
IF protocol for PPIL6 antibody 17452-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Curr Biol

CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement

Authors - Chao Liu
View Article
mouseWB

Cell Mol Life Sci

CCDC146 is required for sperm flagellum biogenesis and male fertility in mice

Authors - Yanjie Ma
View Article