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验证数据展示

  • WB analysis using 31296-1-AP

    WB analysis using 31296-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31296-1-AP (PPL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skin using 31296-1-AP

    IHC staining of mouse skin using 31296-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 31296-1-AP (PPL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skin using 31296-1-AP

    IHC staining of mouse skin using 31296-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 31296-1-AP (PPL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat skin using 31296-1-AP

    IHC staining of rat skin using 31296-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 31296-1-AP (PPL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat skin using 31296-1-AP

    IHC staining of rat skin using 31296-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 31296-1-AP (PPL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HaCaT using 31296-1-AP

    IF Staining of HaCaT using 31296-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HaCaT cells using PPL antibody (31296-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

PPL Polyclonal antibody

PPL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31296-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

periplakin, KIAA0568

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, mouse skin tissue, rat skin tissue
Positive IHC detected inmouse skin tissue, rat skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHaCaT cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31296-1-AP targets PPL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag34952

Product name: Recombinant human PPL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 50-250 aa of BC114620

Sequence: AKMQSDLARLQEGRQPEHRDVTLQKVLDSEKLLYVLEADAAIAKHMKHPQGDMIAEDIRQLKERVTNLRGKHKQIYRLAVKEVDPQVNWAALVEEKLDKLNNQSFGTDLPLVDHQVEEHNIFHNEVKAIGPHLAKDGDKEQNSELRAKYQKLLAASQARQQHLSSLQDYMQRCTNELYWLDQQAKGRMQYDWSDRNLDYPS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 periplakin
别名 periplakin, KIAA0568
观测分子量205 kDa
GenBank蛋白编号BC114620
基因名称 PPL
Gene ID (NCBI) 5493
RRIDAB_3669933
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO60437
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Periplakin (PPL) is a main member in plakin family, which serve as epidermal cytolinkers and components of cell-cell and cell-matrix adhesion complexes, i.e., desmosomes and hemidesmosomes, respectively (PMID: 35612641). Periplakin participates with dynamic changes in the keratin cytoskeleton via its C-terminal linker domain that co-localises with okadaic-acid-treated keratin granules (PMID: 17158917).

实验方案

Product Specific Protocols
IF protocol for PPL antibody 31296-1-APDownload protocol
IHC protocol for PPL antibody 31296-1-APDownload protocol
WB protocol for PPL antibody 31296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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