• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 31296-1-AP

    WB analysis using 31296-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31296-1-AP (PPL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skin using 31296-1-AP

    IHC staining of mouse skin using 31296-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 31296-1-AP (PPL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skin using 31296-1-AP

    IHC staining of mouse skin using 31296-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 31296-1-AP (PPL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat skin using 31296-1-AP

    IHC staining of rat skin using 31296-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 31296-1-AP (PPL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat skin using 31296-1-AP

    IHC staining of rat skin using 31296-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 31296-1-AP (PPL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HaCaT using 31296-1-AP

    IF Staining of HaCaT using 31296-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HaCaT cells using PPL antibody (31296-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

PPL Polyclonal antibody

PPL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31296-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

periplakin, KIAA0568

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA431 cells, mouse skin tissue, rat skin tissue
Positive IHC detected inmouse skin tissue, rat skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHaCaT cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31296-1-AP targets PPL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PPL fusion protein Ag34952 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name periplakin
Synonyms periplakin, KIAA0568
Observed Molecular Weight205 kDa
GenBank Accession NumberBC114620
Gene Symbol PPL
Gene ID (NCBI) 5493
RRIDAB_3669933
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO60437
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Periplakin (PPL) is a main member in plakin family, which serve as epidermal cytolinkers and components of cell-cell and cell-matrix adhesion complexes, i.e., desmosomes and hemidesmosomes, respectively (PMID: 35612641). Periplakin participates with dynamic changes in the keratin cytoskeleton via its C-terminal linker domain that co-localises with okadaic-acid-treated keratin granules (PMID: 17158917).

实验方案

Product Specific Protocols
WB protocol for PPL antibody 31296-1-APDownload protocol
IHC protocol for PPL antibody 31296-1-APDownload protocol
IF protocol for PPL antibody 31296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols