• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of MCF-7 using 26532-1-AP

    IF Staining of MCF-7 using 26532-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 26532-1-AP (PPM1D antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis of MCF-7 using 26532-1-AP

    WB analysis of MCF-7 using 26532-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26532-1-AP (PPM1D antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

PPM1D Polyclonal antibody

PPM1D Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26532-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, Rat

应用

WB, IF/ICC, ELISA

别名

PP2C DELTA, PPM1D, Protein phosphatase 1D, WIP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inMCF-7 cells, mouse lung tissue, rat testis tissue
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PPM1D Ready-To-Use IHC Kit. PPM1D primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26532-1-AP targets PPM1D in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, Rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, Rat
免疫原 PPM1D fusion protein Ag24751 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 1D magnesium-dependent, delta isoform
别名 PP2C DELTA, PPM1D, Protein phosphatase 1D, WIP1
观测分子量 67 kDa
GenBank蛋白编号BC060877
基因名称 PPM1D
Gene ID (NCBI) 8493
RRIDAB_2880544
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15297
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPM1D (also known as PP2Cd and WIP1) is a member of the PPM family, which is characterized by Mg2+/Mn2+-dependent activity and relative insensitivity to okadaic acid. The PPM1D gene is aberrantly amplified in a range of common cancers and encodes a protein phosphatase that is a potential therapeutic target (PMID: 17700519). This antibody can be detected two bands of 47 and 67 Kda.

实验方案

Product Specific Protocols
WB protocol for PPM1D antibody 26532-1-APDownload protocol
IF protocol for PPM1D antibody 26532-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols