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验证数据展示

  • WB analysis using 25089-1-AP

    WB analysis using 25089-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25089-1-AP (PPM1E antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 25089-1-AP

    IHC staining of rat brain using 25089-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25089-1-AP (PPM1E antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 25089-1-AP

    IF Staining of A431 using 25089-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PPM1E antibody (25089-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of SH-SY5Y using 25089-1-AP

    IF Staining of SH-SY5Y using 25089-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using PPM1E antibody (25089-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

PPM1E Polyclonal antibody

PPM1E Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25089-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

EC:3.1.3.16, CaMKP-nucleus, CaMKP-N, CaMKP N, CaMKN

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells, A431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25089-1-AP targets PPM1E in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PPM1E fusion protein Ag18751 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 1E (PP2C domain containing)
别名 EC:3.1.3.16, CaMKP-nucleus, CaMKP-N, CaMKP N, CaMKN
计算分子量 764 aa, 85 kDa
观测分子量85 kDa
GenBank蛋白编号BC136290
基因名称 PPM1E
Gene ID (NCBI) 22843
RRIDAB_3085770
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WY54
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ca2+/calmodulin-dependent protein kinase phosphatase (CaMKP-N),also known as Ppm1E, is an AMPKα phosphatase,which is up-regulated in human gastric cancer tissues (PMID: 28423719).

实验方案

Product Specific Protocols
WB protocol for PPM1E antibody 25089-1-APDownload protocol
IHC protocol for PPM1E antibody 25089-1-APDownload protocol
IF protocol for PPM1E antibody 25089-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols