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  • Featured Product
  • KD/KO Validated

PPM1F Polyclonal antibody

PPM1F Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17020-1-AP

Print datasheet

Synonyms

FEM-2, EC:3.1.3.16, CaMKPase, CaM-kinase phosphatase, CaM kinase phosphatase

验证数据展示

  • WB analysis of MCF-7 using 17020-1-AP

    WB analysis of MCF-7 using 17020-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 17020-1-AP

    IP experiment of MCF-7 using 17020-1-AP

    IP result of anti-PPM1F (IP:17020-1-AP, 4ug; Detection:17020-1-AP 1:700) with MCF-7 cells lysate 2800ug.

  • IF Staining of HepG2 using 17020-1-AP

    IF Staining of HepG2 using 17020-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 17020-1-AP (PPM1F antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 17020-1-AP

    WB analysis using 17020-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 17020-1-AP

    IHC staining of human colon cancer using 17020-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17020-1-AP (PPM1F antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 17020-1-AP

    IHC staining of human ovary cancer using 17020-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17020-1-AP (PPM1F antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMCF-7 cells, L02 cells, HepG2 cells, human liver tissue, K-562 cells, HeLa cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman ovary cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17020-1-AP targets PPM1F in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen PPM1F fusion protein Ag10186 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 1F (PP2C domain containing)
Synonyms FEM-2, EC:3.1.3.16, CaMKPase, CaM-kinase phosphatase, CaM kinase phosphatase
Calculated Molecular Weight 453 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC071989
Gene Symbol PPM1F
Gene ID (NCBI) 9647
RRIDAB_2284338
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49593
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mg2+/Mn2+-dependent protein phosphatase 1F (PPM1F) belongs to the PP2C family of Ser/Thr protein phosphatases and is the critical enzyme responsible for dephosphorylating the threonine motif. PPM1F is ubiquitous in various tissues and organs (PMID: 26498692). High expression of PPM1F was positively associated with poor survival and tumor recurrence in patients with cancers and PPM1F might be a potential biomarker in HCC patients (PMID: 30470261).

实验方案

Product Specific Protocols
WB protocol for PPM1F antibody 17020-1-APDownload protocol
IHC protocol for PPM1F antibody 17020-1-APDownload protocol
IF protocol for PPM1F antibody 17020-1-APDownload protocol
IP protocol for PPM1F antibody 17020-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Cell Biol

PPM1F controls integrin activity via a conserved phospho-switch.

Authors - Tanja M Grimm
  • KO Validated
View Article
humanWB

Clin Transl Oncol

PPM1F regulates ovarian cancer progression by affecting the dephosphorylation of ITGB1

Authors - Yahui Leng
View Article