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验证数据展示

  • WB analysis of mouse kidney using 18203-1-AP

    WB analysis of mouse kidney using 18203-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18203-1-AP (PPM1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 18203-1-AP

    IHC staining of human kidney using 18203-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18203-1-AP (PPM1L Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 18203-1-AP

    IHC staining of human kidney using 18203-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18203-1-AP (PPM1L Antibody) at dilution of 1:50 (under 40x lens).

PPM1L Polyclonal antibody

PPM1L Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18203-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

PP2C epsilon, PP2CE, PPM1 LIKE, PPM1L, Protein phosphatase 1 like, Protein phosphatase 1L

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

18203-1-AP targets PPM1L in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 PPM1L fusion protein Ag12775 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 1 (formerly 2C)-like
别名 PP2C epsilon, PP2CE, PPM1 LIKE, PPM1L, Protein phosphatase 1 like, Protein phosphatase 1L
计算分子量 26 kDa, 41 kDa
观测分子量 41-45 kDa
GenBank蛋白编号BC104885
基因名称 PPM1L
Gene ID (NCBI) 151742
RRIDAB_10859821
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5SGD2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPM1L(Protein phosphatase 1L) is also named as PP2CE.It belongs to the PP2C group of serine/threonine phosphatases, which are distinguished from other phosphatases by their structure, absolute requirement for Mg(2+) or Mn(2+), and insensitivity to okadaic acid. It has 4 isoforms produced by alternative splicing.This antibody is specific to PPM1L.

实验方案

Product Specific Protocols
WB protocol for PPM1L antibody 18203-1-APDownload protocol
IHC protocol for PPM1L antibody 18203-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Dis

Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration

Authors - Xiaoman Zhu
View Article