PPP1CA Polyclonal antibody
PPP1CA Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, LNCaP cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
28617-1-AP targets PPP1CA in WB, IHC, ELISA applications and shows reactivity with Human samples.
Tested Applications | WB, IHC, ELISA Application Description |
Tested Reactivity | Human |
Immunogen | PPP1CA fusion protein Ag28451 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | protein phosphatase 1, catalytic subunit, alpha isoform |
Synonyms | PP 1A, PPP1A, PPP1CA |
Calculated Molecular Weight | 330 aa, 38 kDa |
Observed Molecular Weight | 37 kDa |
GenBank Accession Number | BC001888 |
Gene Symbol | PPP1CA |
Gene ID (NCBI) | 5499 |
RRID | AB_2918182 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P62136 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for PPP1CA antibody 28617-1-AP | Download protocol |
IHC protocol for PPP1CA antibody 28617-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |