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验证数据展示

  • WB analysis using 30070-1-AP

    WB analysis using 30070-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30070-1-AP (PPP1CC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 30070-1-AP

    IHC staining of mouse heart using 30070-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 30070-1-AP (PPP1CC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPP1CC antibody included
  • IHC staining of mouse heart using 30070-1-AP

    IHC staining of mouse heart using 30070-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 30070-1-AP (PPP1CC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPP1CC antibody included
  • IF Staining of HeLa using 30070-1-AP

    IF Staining of HeLa using 30070-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PPP1CC antibody (30070-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

PPP1CC Polyclonal antibody

PPP1CC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30070-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

PP 1G, PPP1CC, PPP1G

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PPP1CC Ready-To-Use IHC Kit. PPP1CC primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30070-1-AP targets PPP1CC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 PPP1CC fusion protein Ag32348 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 1, catalytic subunit, gamma isoform
别名 PP 1G, PPP1CC, PPP1G
计算分子量 35 kDa
观测分子量 35 kDa
GenBank蛋白编号BC014073
基因名称 PPP1CC
Gene ID (NCBI) 5501
RRIDAB_3086223
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP36873
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP1CC, also named as PP-1G, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. It is involved in regulation of ionic conductances and long-term synaptic plasticity. PPP1CC may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II.

实验方案

Product Specific Protocols
WB protocol for PPP1CC antibody 30070-1-APDownload protocol
IHC protocol for PPP1CC antibody 30070-1-APDownload protocol
IF protocol for PPP1CC antibody 30070-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols