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验证数据展示

  • WB analysis using 68122-1-Ig

    WB analysis using 68122-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68122-1-Ig (PPP1CC antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IF Staining of C2C12 using 68122-1-Ig

    IF Staining of C2C12 using 68122-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using PPP1CC antibody (68122-1-Ig, Clone: 2G11C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of HeLa using 68122-1-Ig

    IF Staining of HeLa using 68122-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PPP1CC antibody (68122-1-Ig, Clone: 2G11C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

PPP1CC Monoclonal antibody

PPP1CC Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68122-1-Ig

产品说明书

CloneNo. 2G11C3

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

PP 1G, PPP1CC, PPP1G

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells, C2C12 cells
Planning an IHC experiment? We recommend our IHCeasy PPP1CC Ready-To-Use IHC Kit. PPP1CC primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68122-1-Ig targets PPP1CC in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 PPP1CC fusion protein Ag32348 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein phosphatase 1, catalytic subunit, gamma isoform
别名 PP 1G, PPP1CC, PPP1G
计算分子量 35 kDa
观测分子量35kDa
GenBank蛋白编号BC014073
基因名称 PPP1CC
Gene ID (NCBI) 5501
RRIDAB_2923650
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP36873
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP1CC, also named as PP-1G, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. It is involved in regulation of ionic conductances and long-term synaptic plasticity. PPP1CC may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II.

实验方案

Product Specific Protocols
WB protocol for PPP1CC antibody 68122-1-IgDownload protocol
IF protocol for PPP1CC antibody 68122-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols