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PPP1R12C Polyclonal antibody

PPP1R12C Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25157-1-AP

Print datasheet

Synonyms

Protein phosphatase 1 myosin-binding subunit p85, Protein phosphatase 1 myosin-binding subunit of 85 kDa, p84, MBS85, LENG3

验证数据展示

  • WB analysis using 25157-1-AP

    WB analysis using 25157-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25157-1-AP (PPP1R12C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse colon tissue, HEK-293T cells, mouse heart tissue, rat heart tissue, rat colon tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25157-1-AP targets PPP1R12C in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PPP1R12C fusion protein Ag18559 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 12C
Synonyms Protein phosphatase 1 myosin-binding subunit p85, Protein phosphatase 1 myosin-binding subunit of 85 kDa, p84, MBS85, LENG3
Calculated Molecular Weight 782 aa, 85 kDa
Observed Molecular Weight90-100 kDa
GenBank Accession NumberBC008030
Gene Symbol PPP1R12C
Gene ID (NCBI) 54776
RRIDAB_3669464
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZL4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP1R12C is a subunit of myosin phosphatase. PPP1R12C regulates the catalytic activity of protein phosphatase 1 delta and assembly of the actin cytoskeleton.

实验方案

Product Specific Protocols
WB protocol for PPP1R12C antibody 25157-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols