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PPP1R13B Polyclonal antibody

PPP1R13B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29804-1-AP

Print datasheet

Synonyms

ASPP1, KIAA0771, p53BP2 like, p85, PPP1R13B

验证数据展示

  • WB analysis using 29804-1-AP

    WB analysis using 29804-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29804-1-AP (PPP1R13B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 29804-1-AP

    IHC staining of human lung cancer using 29804-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29804-1-AP (PPP1R13B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 29804-1-AP

    IHC staining of human lung cancer using 29804-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29804-1-AP (PPP1R13B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, MCF-7 cells
Positive IHC detected inhuman lung cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

29804-1-AP targets PPP1R13B in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, Mouse
Cited Reactivitysheep
Immunogen PPP1R13B fusion protein Ag30788 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 13B
Synonyms ASPP1, KIAA0771, p53BP2 like, p85, PPP1R13B
Calculated Molecular Weight 1090 aa, 120 kDa
Observed Molecular Weight120 kDa
GenBank Accession NumberBC136527
Gene Symbol PPP1R13B
Gene ID (NCBI) 23368
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96KQ4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP1R13B(Protein phosphatase 1 regulatory subunit 13B), also named ASPP1 and KIAA0771, is a member of the apoptosis-stimulating proteins of the p53 family (ASPPs)(PMID: 30908929). PPP1R13B contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. PPP1R13 promotes DNA binding and transactivation of p53-family proteins on the promoters of proapoptotic genes. Expression of this gene is regulated by the E2F transcription factor.

实验方案

Product Specific Protocols
WB protocol for PPP1R13B antibody 29804-1-APDownload protocol
IHC protocol for PPP1R13B antibody 29804-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
sheepWB

Int J Biol Macromol

Estrogen-mediated oar-miR-485-5p targets PPP1R13B to regulate myoblast proliferation in sheep

Authors - Siqi Liu
View Article