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  • KD/KO Validated

iASPP Polyclonal antibody

iASPP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 18590-1-AP

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Synonyms

PPP1R13L, PPP1R13B-like protein, PPP1R13BL, PPP1R13B like protein, NKIP1



经过测试的应用

Positive WB detected inNIH/3T3 cells, PC-3 cells, MCF-7 cells, Apoptosised HeLa cells, C6 cells
Positive IP detected inPC-3 cells
Positive IHC detected inhuman breast cancer tissue, human cervical squamous cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PPP1R13L Ready-To-Use IHC Kit. PPP1R13L primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18590-1-AP targets iASPP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen iASPP fusion protein Ag13273 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 13 like
Synonyms PPP1R13L, PPP1R13B-like protein, PPP1R13BL, PPP1R13B like protein, NKIP1
Calculated Molecular Weight 89 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC064913
Gene Symbol PPP1R13L
Gene ID (NCBI) 10848
RRIDAB_2168573
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WUF5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Inhibitor of apoptosis-stimulating protein of p53 (iASPP), encoded by PPP1R13L gene, is often overexpressed in human cancers. The ASPP family includes three members, namely ASPP1, ASPP2, and iASPP, which are specific regulators of p53-, p63-, and p73-mediated apoptosis. ASPP1 and ASPP2 enhance the apoptotic function of p53, whereas iASPP specifically inhibits p53- mediated apoptosis. Overexpression of iASPP is associated with resistance to cisplatin-induced apoptosis and rediation therapy. iASPP plays a pivotal role in regulating cancer cell proliferation and tumor progression. This antibody could both recognize unphosphorylated and phosphorylated iASPP.

实验方案

Product Specific Protocols
WB protocol for iASPP antibody 18590-1-APDownload protocol
IHC protocol for iASPP antibody 18590-1-APDownload protocol
IF protocol for iASPP antibody 18590-1-APDownload protocol
IP protocol for iASPP antibody 18590-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

J Cell Biol

iASPP contributes to cell cortex rigidity, mitotic cell rounding, and spindle positioning.

Authors - Aurélie Mangon
  • KD Validated
humanWB,IP,IF

Cell Death Dis

iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.

Authors - Kun Gao
  • KD Validated
mouseWB

J Biochem Mol Toxicol

Upregulation of iASPP ameliorates hypoxia/reoxygenation-induced apoptosis and oxidative stress in cardiomyocytes by upregulating Nrf2 signaling.

Authors - Baobao Bai
  • KD Validated
mouseWB,IF

Metab Brain Dis

Inhibitor of apoptosis stimulating protein of p53 protects against MPP+-induced neurotoxicity of dopaminergic neurons

Authors - Lei Chen
mouseWB

Acta Biochim Biophys Sin (Shanghai)

iASPP protects the heart from ischemia injury by inhibiting p53 expression and cardiomyocyte apoptosis.

Authors - Timur Yagudin