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iASPP Polyclonal antibody
iASPP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | NIH/3T3 cells, PC-3 cells, MCF-7 cells, Apoptosised HeLa cells, C6 cells |
Positive IP detected in | PC-3 cells |
Positive IHC detected in | human breast cancer tissue, human cervical squamous cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
18590-1-AP targets iASPP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IF, IP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | iASPP fusion protein Ag13273 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | protein phosphatase 1, regulatory (inhibitor) subunit 13 like |
Synonyms | PPP1R13L, PPP1R13B-like protein, PPP1R13BL, PPP1R13B like protein, NKIP1 |
Calculated Molecular Weight | 89 kDa |
Observed Molecular Weight | 110 kDa |
GenBank Accession Number | BC064913 |
Gene Symbol | PPP1R13L |
Gene ID (NCBI) | 10848 |
RRID | AB_2168573 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q8WUF5 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for iASPP antibody 18590-1-AP | Download protocol |
IHC protocol for iASPP antibody 18590-1-AP | Download protocol |
IF protocol for iASPP antibody 18590-1-AP | Download protocol |
IP protocol for iASPP antibody 18590-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Cell Biol iASPP contributes to cell cortex rigidity, mitotic cell rounding, and spindle positioning.
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Cell Death Dis iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.
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J Biochem Mol Toxicol Upregulation of iASPP ameliorates hypoxia/reoxygenation-induced apoptosis and oxidative stress in cardiomyocytes by upregulating Nrf2 signaling.
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Metab Brain Dis Inhibitor of apoptosis stimulating protein of p53 protects against MPP+-induced neurotoxicity of dopaminergic neurons | ||
Acta Biochim Biophys Sin (Shanghai) iASPP protects the heart from ischemia injury by inhibiting p53 expression and cardiomyocyte apoptosis. |