验证数据展示
经过测试的应用
| Positive WB detected in | human heart tissue, HEK-293 cells, mouse testis tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy PPP1R13L Ready-To-Use IHC Kit. PPP1R13L primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
51141-1-AP targets PPP1R13L in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | PPP1R13L fusion protein Ag0635 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | protein phosphatase 1, regulatory (inhibitor) subunit 13 like |
| Synonyms | IASPP, Inhibitor of ASPP protein, NFkB interacting protein 1, NKIP1, PPP1R13B like protein, PPP1R13BL, PPP1R13L, Protein iASPP, RAI, RelA associated inhibitor |
| Calculated Molecular Weight | 45 kDa, 89 kDa |
| Observed Molecular Weight | 110 kDa |
| GenBank Accession Number | BC032298 |
| Gene Symbol | PPP1R13L |
| Gene ID (NCBI) | 10848 |
| RRID | AB_2268619 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WUF5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPP1R13L antibody 51141-1-AP | Download protocol |
| IHC protocol for PPP1R13L antibody 51141-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Med Exome-wide evaluation of rare coding variants using electronic health records identifies new gene-phenotype associations. | ||
Adv Sci (Weinh) Chitosan-Gelatin-EGCG Nanoparticle-Meditated LncRNA TMEM44-AS1 Silencing to Activate the P53 Signaling Pathway for the Synergistic Reversal of 5-FU Resistance in Gastric Cancer
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