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PPP1R15B Recombinant antibody
PPP1R15B Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
240052G5
验证数据展示
经过测试的应用
| Positive WB detected in | SGC-7901 cells, A2780 cells, MDA-MB-231 cells, SKOV-3 cells |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | U2OS cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 83016-6-RR is PPP1R15B Fusion Protein expressed in E. coli.
| Tested Applications | WB, IF/ICC, FC (Intra), ELISA Application Description |
| Tested Reactivity | human |
| Immunogen | PPP1R15B fusion protein Ag6213 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | protein phosphatase 1, regulatory (inhibitor) subunit 15B |
| Synonyms | FLJ14744, CREP, 240052G5 |
| Calculated Molecular Weight | 79 kDa |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | BC065280 |
| Gene Symbol | PPP1R15B |
| Gene ID (NCBI) | 84919 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q5SWA1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein phosphatase 1 regulatory subunit 15B(PPP1R15B) is a member of PPP1R15 family, it forms a complex with protein phosphatase1(PP1) and NCK1/2, maintaining low levels of EIF2S1 phosphorylation in unstressed cells, thus is also named constitutive repressor of eIF2alpha phosphorylation (CReP). PPP1R15B is assoicated in endoplasmic reticulum (ER) stress response resulting to a role in translation regulation. Knock-down of PPP1R15B strongly protected mammalian cells against oxidative stress, peroxynitrite stress, and more modestly against accumulation of malfolded proteins in the ER. Recently, PPP1R15B was found to associate with cell membranes and regulate membrane traffic in a PP1c-independent manner, suggesting a novel link between translation and traffic. MW of PPP1R15B is 80 kDa. Observed MW of PPP1R15B is from 100-110 kDa maybe due to phosphorylation (PMID: 28492545).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPP1R15B antibody 83016-6-RR | Download protocol |
| IF protocol for PPP1R15B antibody 83016-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
