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PPP1R2P9 Polyclonal antibody

PPP1R2P9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11969-1-AP

Print datasheet

Synonyms

验证数据展示

  • IHC staining of mouse testis using 11969-1-AP

    IHC staining of mouse testis using 11969-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11969-1-AP (PPP1R2P9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 11969-1-AP

    IHC staining of mouse testis using 11969-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11969-1-AP (PPP1R2P9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human testis using 11969-1-AP

    WB analysis of human testis using 11969-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 11969-1-AP (PPP1R2P9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 11969-1-AP

    IF Staining of HepG2 using 11969-1-AP

    Immunofluorescent analysis of HepG2 cells, using PPP1R2P9 antibody 11969-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inhuman testis tissue, human brain tissue
Positive IHC detected inmouse testis tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11969-1-AP targets PPP1R2P9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PPP1R2P9 fusion protein Ag2591 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 2 pseudogene 9
Synonyms
Calculated Molecular Weight 202 aa, 23 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC056673
Gene Symbol PPP1R2P9
Gene ID (NCBI) 80316
RRIDAB_10666997
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPP1R2P9 antibody 11969-1-APDownload protocol
IHC protocol for PPP1R2P9 antibody 11969-1-APDownload protocol
IF protocol for PPP1R2P9 antibody 11969-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols