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验证数据展示

  • WB analysis using 84155-2-RR

    WB analysis using 84155-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84155-2-RR (PPP2CA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84155-2-RR

    Affinity and Kinetic Characterization of 84155-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84155-2-RR against Human PPP2CA were performed. The affinity constant is 11.6 nM.

PPP2CA Recombinant antibody

PPP2CA Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84155-2-RR

Print datasheet

CloneNo. 241112A6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

PP2CA, PP2Ac, PP2A-alpha, PP2A alpha, EC:3.1.3.16

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, HEK-293T cells, MCF-7 cells, mouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy PPP2CA Ready-To-Use IHC Kit. PPP2CA primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84155-2-RR targets PPP2CA in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PPP2CA fusion protein Ag4294 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
Synonyms PP2CA, PP2Ac, PP2A-alpha, PP2A alpha, EC:3.1.3.16
Calculated Molecular Weight 309 aa, 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC031696
Gene Symbol PPP2CA
Gene ID (NCBI) 5515
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP67775
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.

实验方案

Product Specific Protocols
WB protocol for PPP2CA antibody 84155-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols