PPP2CA Recombinant antibody

PPP2CA Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 84155-6-RR

产品说明书

CloneNo. 241112F3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

PP2CA, PP2Ac, PP2A-alpha, PP2A alpha, EC:3.1.3.16

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PPP2CA Ready-To-Use IHC Kit. PPP2CA primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84155-6-RR targets PPP2CA in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 PPP2CA fusion protein Ag4294 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
别名 PP2CA, PP2Ac, PP2A-alpha, PP2A alpha, EC:3.1.3.16
计算分子量 309 aa, 36 kDa
GenBank蛋白编号BC031696
基因名称 PPP2CA
Gene ID (NCBI) 5515
RRIDAB_3671720
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP67775
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.

实验方案

Product Specific Protocols
IF protocol for PPP2CA antibody 84155-6-RRDownload protocol
FC protocol for PPP2CA antibody 84155-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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