验证数据展示
经过测试的应用
Positive IF/ICC detected in | MCF-7 cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84155-6-RR targets PPP2CA in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
经测试应用 | IF/ICC, FC (Intra), ELISA Application Description |
经测试反应性 | human |
免疫原 | PPP2CA fusion protein Ag4294 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform |
别名 | PP2CA, PP2Ac, PP2A-alpha, PP2A alpha, EC:3.1.3.16 |
计算分子量 | 309 aa, 36 kDa |
GenBank蛋白编号 | BC031696 |
基因名称 | PPP2CA |
Gene ID (NCBI) | 5515 |
RRID | AB_3671720 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | P67775 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.
实验方案
Product Specific Protocols | |
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IF protocol for PPP2CA antibody 84155-6-RR | Download protocol |
FC protocol for PPP2CA antibody 84155-6-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |