• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

PPP2CB Polyclonal antibody

PPP2CB Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12554-2-AP

Print datasheet

Synonyms

Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform, PP2CB, PP2A-beta, PP2A beta, EC:3.1.3.16

验证数据展示

  • WB analysis of mouse lung using 12554-2-AP

    WB analysis of mouse lung using 12554-2-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 12554-2-AP (PPP2CB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IP experiment of NIH/3T3 using 12554-2-AP

    IP experiment of NIH/3T3 using 12554-2-AP

    IP result of anti-PPP2CB (IP:12554-2-AP, 4ug; Detection:12554-2-AP 1:300) with NIH/3T3 cells lysate 4000ug.

  • IHC staining of human stomach cancer using 12554-2-AP

    IHC staining of human stomach cancer using 12554-2-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12554-2-AP (PPP2CB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

  • IHC staining of rat kidney using 12554-2-AP

    IHC staining of rat kidney using 12554-2-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 12554-2-AP (PPP2CB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse lung tissue, U2OS cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inrat kidney tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12554-2-AP targets PPP2CB in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen PPP2CB fusion protein Ag3233 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform
Synonyms Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform, PP2CB, PP2A-beta, PP2A beta, EC:3.1.3.16
Calculated Molecular Weight 309 aa, 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC012022
Gene Symbol PPP2CB
Gene ID (NCBI) 5516
RRIDAB_2169651
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62714
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPP2CB antibody 12554-2-APDownload protocol
IHC protocol for PPP2CB antibody 12554-2-APDownload protocol
IP protocol for PPP2CB antibody 12554-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,CoIP

Cell Mol Life Sci

GADD45α alleviates acetaminophen-induced hepatotoxicity by promoting AMPK activation.

Authors - Chunmin Li
  • KD Validated
View Article
humanWB

Front Cell Dev Biol

BRG1 Activates PR65A Transcription to Regulate NO Bioavailability in Vascular Endothelial Cells.

Authors - Baoyu Chen
View Article
humanWB,CoIP

Cell Signal

METTL14-mediated N6-methyladenosine modification of TCP1 mRNA promotes acute myeloid leukemia progression

Authors - Mengmeng Zhang
View Article