验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, SH-SY5Y cells, MCF-7 cells, mouse brain tissue, HepG2 cells, fetal human brain tissue, rat brain tissue, Jurkat cells |
| Positive IP detected in | SH-SY5Y cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13123-1-AP targets PPP2R2B/A/C/D in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | PPP2R2B/A/C/D fusion protein Ag3860 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform |
| 别名 | PPP2R2B, PP2A subunit B isoform beta, PP2A subunit B isoform B55-beta, PP2A PR55B, PP2A B55BETA |
| 计算分子量 | 443 aa, 52 kDa |
| 观测分子量 | 52 kDa |
| GenBank蛋白编号 | BC031790 |
| 基因名称 | PPP2R2B |
| Gene ID (NCBI) | 5521 |
| RRID | AB_2169964 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q00005 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The PPP2R2B gene encodes a deduced 443-amino acid protein of approximately 52 kDa,which is a brain-specific regulatory subunit B of protein phosphatase 2. PPP2R2B is a Subunit of PP2A, a highly conserved constitutive enzyme(PMID:11719278). PPP2R2B (Bβ) is an important regulator of protein phosphatase 2A (PP2A) activity in the brain. Through differential promoter usage and alternative splicing, two major isoforms Bβ1 and Bβ2 with divergent sub-cellular targeting N termini are produced. Bβ plays an important role in neuronal survival. It has 5 isoforms produced by alternative splicing. This antibody has cross-reactivity to PPP2R2A, PPP2R2C and PPP2R2D.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPP2R2B/A/C/D antibody 13123-1-AP | Download protocol |
| IHC protocol for PPP2R2B/A/C/D antibody 13123-1-AP | Download protocol |
| IF protocol for PPP2R2B/A/C/D antibody 13123-1-AP | Download protocol |
| IP protocol for PPP2R2B/A/C/D antibody 13123-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Ther Nucleic Acids miR21 modulates the Hippo signaling pathway via interference with PP2A Bβ to inhibit trophoblast invasion and cause preeclampsia | ||
Cancer Cell Int PPP2R2B downregulation is associated with immune evasion and predicts poor clinical outcomes in triple-negative breast cancer. |
