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验证数据展示

  • WB analysis of mouse brain using 24877-1-AP

    WB analysis of mouse brain using 24877-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24877-1-AP (PPP2R3C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 24877-1-AP

    IHC staining of human brain using 24877-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24877-1-AP (PPP2R3C Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 24877-1-AP

    IHC staining of human brain using 24877-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24877-1-AP (PPP2R3C Antibody) at dilution of 1:50 (under 40x lens).

PPP2R3C Polyclonal antibody

PPP2R3C Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24877-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma, Rhabdomyosarcoma antigen MU-RMS-40.6A/6C, G5PR, G4 1, C14orf10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman brain tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24877-1-AP targets PPP2R3C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PPP2R3C fusion protein Ag19282 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 2 (formerly 2A), regulatory subunit B'', gamma
别名 Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma, Rhabdomyosarcoma antigen MU-RMS-40.6A/6C, G5PR, G4 1, C14orf10
计算分子量 453 aa, 53 kDa
观测分子量 53 kDa
GenBank蛋白编号BC063438
基因名称 PPP2R3C
Gene ID (NCBI) 55012
RRIDAB_2879773
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ969Q6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP2R3C(Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma) is also named as C14orf10, and G5PR. It may regulate MCM3AP phosphorylation through phosphatase recruitment and play a role in the activation-induced cell death of B-cells. It has 2 isoforms produced by alternative splicing with the molecular weight of 53 kDa and 40 kDa.

实验方案

Product Specific Protocols
WB protocol for PPP2R3C antibody 24877-1-APDownload protocol
IHC protocol for PPP2R3C antibody 24877-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols