- Featured Product
- KD/KO Validated
PPP2R5A Polyclonal antibody
PPP2R5A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | human ileum tissue, HeLa cells, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, mouse heart tissue, rat heart tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:300-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
产品信息
12675-2-AP targets PPP2R5A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | PPP2R5A fusion protein Ag3360 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | protein phosphatase 2, regulatory subunit B', alpha isoform |
| Synonyms | PP2A B subunit isoform R5-alpha, PP2A B subunit isoform PR61-alpha, PP2A B subunit isoform B'-alpha, PP2A B subunit isoform B56-alpha, PP2A B subunit isoform B alpha |
| Calculated Molecular Weight | 486 aa, 56 kDa |
| Observed Molecular Weight | 56 kDa |
| GenBank Accession Number | BC022474 |
| Gene Symbol | PPP2R5A |
| Gene ID (NCBI) | 5525 |
| RRID | AB_2252956 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15172 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PPP2R5A(protein phosphatase 2, regulatory subunit B (B56), alpha isoform) is also named as PR61alpha and belongs to the phosphatase 2A regulatory subunit B56 family. B56 alpha subunit gene encodes a cytoplasmic phosphoprotein(PMID:7592815). The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 54 kDa and 50 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPP2R5A antibody 12675-2-AP | Download protocol |
| IHC protocol for PPP2R5A antibody 12675-2-AP | Download protocol |
| IF protocol for PPP2R5A antibody 12675-2-AP | Download protocol |
| IP protocol for PPP2R5A antibody 12675-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Protein Cell Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer's disease mice | ||
Oncol Lett PP2A promotes apoptosis and facilitates docetaxel sensitivity via the PP2A/p-eIF4B/XIAP signaling pathway in prostate cancer. | ||
Cell Death Dis Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration |
