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验证数据展示

  • IHC staining of mouse liver using 11276-1-AP

    IHC staining of mouse liver using 11276-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11276-1-AP (PPP2R5C Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPP2R5C antibody included
  • IHC staining of mouse liver using 11276-1-AP

    IHC staining of mouse liver using 11276-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11276-1-AP (PPP2R5C Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPP2R5C antibody included
  • WB analysis using 11276-1-AP

    WB analysis using 11276-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11276-1-AP (PPP2R5C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

PPP2R5C Polyclonal antibody

PPP2R5C Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11276-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

B56G, KIAA0044, PP2A B subunit isoform B gamma, PPP2R5C, PR61G

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PPP2R5C Ready-To-Use IHC Kit. PPP2R5C primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11276-1-AP targets PPP2R5C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PPP2R5C fusion protein Ag1819 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 2, regulatory subunit B', gamma isoform
Synonyms B56G, KIAA0044, PP2A B subunit isoform B gamma, PPP2R5C, PR61G
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 53-61 kDa
GenBank Accession NumberBC016183
Gene Symbol PPP2R5C
Gene ID (NCBI) 5527
RRIDAB_3669125
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13362
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPP2R5C antibody 11276-1-APDownload protocol
IHC protocol for PPP2R5C antibody 11276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols