验证数据展示
经过测试的应用
| Positive WB detected in | Y79 cells, human brain tissue |
| Positive IP detected in | Y79 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
产品信息
12068-1-AP targets PPP2R5D in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | PPP2R5D fusion protein Ag2705 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 2, regulatory subunit B', delta isoform |
| 别名 | PP2A B subunit isoform R5-delta, PP2A B subunit isoform PR61-delta, PP2A B subunit isoform B'-delta, PP2A B subunit isoform B56-delta, PP2A B subunit isoform B delta |
| 计算分子量 | 602 aa, 70 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC010692 |
| 基因名称 | PPP2R5D |
| Gene ID (NCBI) | 5528 |
| RRID | AB_2299977 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14738 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPP2R5D antibody 12068-1-AP | Download protocol |
| IHC protocol for PPP2R5D antibody 12068-1-AP | Download protocol |
| IP protocol for PPP2R5D antibody 12068-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO J The CEP170B-KIF2A complex destabilizes microtubule minus ends to generate polarized microtubule network | ||
Biomedicines Unmasking Protein Phosphatase 2A Regulatory Subunit B as a Crucial Factor in the Progression of Dilated Cardiomyopathy
|
