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  • Featured Product
  • KD/KO Validated

PPP3CB Monoclonal antibody, PBS Only

PPP3CB Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C1A3

Cat no : 66614-1-PBS

Print datasheet

Synonyms

CALNA2, CAM-PRP catalytic subunit, CAM PRP catalytic subunit, CALNB, Calmodulin-dependent calcineurin A subunit beta isoform

验证数据展示

  • WB analysis using 66614-1-Ig (same clone as 66614-1-PBS)

    WB analysis using 66614-1-Ig (same clone as 66614-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66614-1-Ig (PPP3CB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66614-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66614-1-Ig (same clone as 66614-1-PBS)

    IF Staining of HeLa using 66614-1-Ig (same clone as 66614-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PPP3CB antibody (66614-1-Ig, Clone: 3C1A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66614-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 66614-1-Ig (same clone as 66614-1-PBS)

    IF Staining of U-251 using 66614-1-Ig (same clone as 66614-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using PPP3CB antibody (66614-1-Ig, Clone: 3C1A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66614-1-PBS in a different storage buffer formulation.

产品信息

66614-1-PBS targets PPP3CB in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen PPP3CB fusion protein Ag26987 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform
Synonyms CALNA2, CAM-PRP catalytic subunit, CAM PRP catalytic subunit, CALNB, Calmodulin-dependent calcineurin A subunit beta isoform
Calculated Molecular Weight 525 aa, 59 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC028049
Gene Symbol PPP3CB
Gene ID (NCBI) 5532
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP16298
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PPP3CB is one of the three isoforms of the A subunit of calcineurin (CnA). Calcineurin is a calcium-dependent serine/threonine phosphatase. It consists of two subunits: subunit A (CnA) and subunit B (CnB). For CnA, three isoforms PPP3CA (CnAα), PPP3CB (CnAβ) and PPP3CC (CnAγ) have been identified. PPP3CB is a calcium-dependent, calmodulin-stimulated protein phosphatase. It may have a role in the calmodulin activation of calcineurin.