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PPP3CC Polyclonal antibody

PPP3CC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19653-1-AP

Print datasheet

Synonyms

CNA3, CAM-PRP catalytic subunit, CAM PRP catalytic subunit, CALNA3, Calmodulin-dependent calcineurin A subunit gamma isoform

验证数据展示

  • WB analysis of mouse testis using 19653-1-AP

    WB analysis of mouse testis using 19653-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 19653-1-AP (PPP3CC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 19653-1-AP

    IHC staining of human stomach using 19653-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 19653-1-AP (PPP3CC antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse testis tissue, human testis tissue, Jurkat cells, MCF-7 cells, mouse brain tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

19653-1-AP targets PPP3CC in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen PPP3CC fusion protein Ag7619 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 3 (formerly 2B), catalytic subunit, gamma isoform
Synonyms CNA3, CAM-PRP catalytic subunit, CAM PRP catalytic subunit, CALNA3, Calmodulin-dependent calcineurin A subunit gamma isoform
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC004864
Gene Symbol PPP3CC
Gene ID (NCBI) 5533
RRIDAB_10695630
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48454
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPP3CC antibody 19653-1-APDownload protocol
IHC protocol for PPP3CC antibody 19653-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

FASEB J

Roles of glycogen synthase kinase 3 alpha and calcineurin in regulating the ability of sperm to fertilize eggs.

Authors - Souvik Dey
View Article
humanWB

BMC Complement Med Ther

Shikonin reactivates TSGs GADD45B and PPP3CC to block NSCLC cell proliferation and migration through JNK/P38/MAPK signaling pathways

Authors - Yujia Zhao
View Article