验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, HeLa cells, human stomach tissue, K-562 cells, rat brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse testis tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy PPP3R1 Ready-To-Use IHC Kit. PPP3R1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
| IF | See 1 publications below |
产品信息
13210-1-AP targets PPP3R1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, chicken |
| 免疫原 | PPP3R1 fusion protein Ag3992 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform |
| 别名 | CNB1, CNB, CNA2, CALNB1, Calcineurin subunit B type 1 |
| 计算分子量 | 170 aa, 19 kDa |
| 观测分子量 | 19 kDa |
| GenBank蛋白编号 | BC027913 |
| 基因名称 | PPP3R1 |
| Gene ID (NCBI) | 5534 |
| RRID | AB_2252760 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P63098 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein phosphatase 3 (PPP3, formerly PP2B, Calcineurin), a serine/ threonine protein phosphatase, is a heterodimer composed of one catalytic subunit (PPP3C, Calcineurin A) and one regulatory subunit (PPP3R, Calcineurin B). PPP3R has two isoforms, PPP3R1 and PPP3R2. PPP3Rl is ubiquitously expressed in different tissues and is coded by the PPP3R1 gene which was mapped recently to human chromosome 2. PPP3Rl has four functional calcium-binding sites.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPP3R1 antibody 13210-1-AP | Download protocol |
| IHC protocol for PPP3R1 antibody 13210-1-AP | Download protocol |
| IP protocol for PPP3R1 antibody 13210-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy SMURF1 controls the PPP3/calcineurin complex and TFEB at a regulatory node for lysosomal biogenesis | ||
Acta Biomater Substrate stiffness regulates differentiation of induced pluripotent stem cells into heart valve endothelial cells. | ||
Proc Natl Acad Sci U S A Major contribution of the 3/6/7 class of TRPC channels to myocardial ischemia/reperfusion and cellular hypoxia/reoxygenation injuries. | ||
Biochem Biophys Res Commun The calcineurin B subunit induces TNF-related apoptosis-inducing ligand (TRAIL) expression via CD11b-NF-κB pathway in RAW264.7 macrophages. | ||
Biol Trace Elem Res Transcriptional Study Revealed That Boron Supplementation May Alter the Immune-Related Genes Through MAPK Signaling in Ostrich Chick Thymus. | ||
Autophagy Narirutin activates TFEB (transcription factor EB) to protect against acetaminophen-induced liver injury by targeting PPP3/calcineurin |
