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  • Featured Product
  • KD/KO Validated

PPP4R1 Polyclonal antibody

PPP4R1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

IHC, IP, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 27805-1-AP

Print datasheet

Synonyms

MEG1, PP4(Rmeg), PP4R1, PPP4R1

验证数据展示

  • WB analysis using 27805-1-AP

    WB analysis using 27805-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27805-1-AP (PPP4R1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 27805-1-AP

    WB analysis of HeLa using 27805-1-AP

    WB result of PPP4R1 antibody (27805-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPP4R1 transfected HeLa cells.

  • IP experiment of HEK-293T using 27805-1-AP

    IP experiment of HEK-293T using 27805-1-AP

    IP result of anti-PPP4R1 (IP:27805-1-AP, 4ug; Detection:27805-1-AP 1:2000) with HEK-293T cells lysate 4000 ug.

  • IHC staining of human liver cancer using 27805-1-AP

    IHC staining of human liver cancer using 27805-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27805-1-AP (PPP4R1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 27805-1-AP

    IHC staining of human liver cancer using 27805-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27805-1-AP (PPP4R1 antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, HEK-293 cells, A431 cells
Positive IP detected inHEK-293T cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27805-1-AP targets PPP4R1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, IP, WB,ELISA Application Description
Cited ApplicationsWB, IP, IF, IHC
Tested Reactivity Human
Cited Reactivityhuman
Immunogen PPP4R1 fusion protein Ag26405 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 4, regulatory subunit 1
Synonyms MEG1, PP4(Rmeg), PP4R1, PPP4R1
Calculated Molecular Weight 107 kDa
Observed Molecular Weight 120-130 kDa
GenBank Accession NumberBC060829
Gene Symbol PPP4R1
Gene ID (NCBI) 9989
RRIDAB_2880978
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TF05
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPP4R1 antibody 27805-1-APDownload protocol
IHC protocol for PPP4R1 antibody 27805-1-APDownload protocol
IP protocol for PPP4R1 antibody 27805-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF,IP

Cancer Lett

PP4R1 promotes glycolysis and gallbladder cancer progression through facilitating ERK1/2 mediated PKM2 nuclear translocation

Authors - Zhiqiang He
View Article
humanWB,IP

Cell Death Differ

AMBRA1 promotes intestinal inflammation by antagonizing PP4R1/PP4c mediated IKK dephosphorylation in an autophagy-independent manner

Authors - Weimin Xu
View Article