验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, HEK-293 cells, human testis tissue, NIH/3T3 cells, rat testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy PPP6C Ready-To-Use IHC Kit. PPP6C primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15852-1-AP targets PPP6C in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, canine |
| 免疫原 | PPP6C fusion protein Ag8670 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 6, catalytic subunit |
| 别名 | PP6C, PPP6, PPP6C |
| 计算分子量 | 305 aa, 35 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC006990 |
| 基因名称 | PPP6C |
| Gene ID (NCBI) | 5537 |
| RRID | AB_2168894 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00743 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPP6C antibody 15852-1-AP | Download protocol |
| IHC protocol for PPP6C antibody 15852-1-AP | Download protocol |
| IP protocol for PPP6C antibody 15852-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Protein Cell Dephosphorylation of cGAS by PPP6C impairs its substrate binding activity and innate antiviral response. | ||
J Biol Chem Leucine Carboxyl Methyltransferase 1 (LCMT-1) Methylates Protein Phosphatase 4 (PP4) and Protein Phosphatase 6 (PP6) and Differentially Regulates the Stable Formation of Different PP4 Holoenzymes. | ||
Cell Death Dis PP6 negatively modulates LUBAC-mediated M1-ubiquitination of RIPK1 and c-FLIPL to promote TNFα-mediated cell death
| ||
Mol Cell Energy sensor AMPK gamma regulates translation via phosphatase PPP6C independent of AMPK alpha | ||
J Vet Med Sci Protein phosphatase 6 regulates trametinib sensitivity, a mitogen-activated protein kinase kinase (MEK) inhibitor, by regulating MEK1/2-ERK1/2 signaling in canine melanoma cells
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