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验证数据展示

  • WB analysis of NIH/3T3 using 25429-1-AP

    WB analysis of NIH/3T3 using 25429-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25429-1-AP (PPRC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis using 25429-1-AP

    WB analysis using 25429-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25429-1-AP (PPRC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

PPRC1 Polyclonal antibody

PPRC1 Polyclonal Antibody for WB, ELISA
Cat No. 25429-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

KIAA0595, PGC 1 related coactivator, PPRC1, PRC, RP11 302K17.6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, C6 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

25429-1-AP targets PPRC1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 PPRC1 fusion protein Ag11940 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peroxisome proliferator-activated receptor gamma, coactivator-related 1
别名 KIAA0595, PGC 1 related coactivator, PPRC1, PRC, RP11 302K17.6
计算分子量 1542 aa, 165 kDa
观测分子量 177 kDa
GenBank蛋白编号BC063806
基因名称 PPRC1
Gene ID (NCBI) 23082
RRIDAB_2880074
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5VV67
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Peroxisome proliferator-activated receptor gamma, co-activator-related 1 (Pprc1) is the third member of the Pgc1 family, which contributes to mitochondrial biogenesis and orchestrates response to metabolic stress by promoting the expression of multiple genes specifying inflammation, proliferation, and metabolic reprogramming.

实验方案

Product Specific Protocols
WB protocol for PPRC1 antibody 25429-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Res

PGC-1α degradation suppresses mitochondrial biogenesis to confer radiation resistance in glioma

Authors - Mengjie Zhao
View Article
humanWB

Sci Rep

CRIP1 inhibits cutaneous melanoma progression through TFAM-mediated mitochondrial biogenesis

Authors - Jianqiang Wu
View Article