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验证数据展示

  • WB analysis using 67699-1-Ig

    WB analysis using 67699-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67699-1-Ig (PPT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 67699-1-Ig

    WB analysis of A549 using 67699-1-Ig

    WB result of PPT1 antibody (67699-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPT1 transfected A549 cells.

  • WB analysis using 67699-1-Ig

    WB analysis using 67699-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67699-1-Ig (PPT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse testis using 67699-1-Ig

    IF Staining of mouse testis using 67699-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using PPT1 antibody (67699-1-Ig, Clone: 1A1A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

PPT1 Monoclonal antibody

PPT1 Monoclonal Antibody for WB, IF-P, ELISA
Cat No. 67699-1-Ig

产品说明书

CloneNo. 1A1A3

宿主/亚型

Mouse / IgG2a

种属反应性

human, pig, rabbit

应用

WB, IF-P, ELISA

别名

Palmitoyl-protein thioesterase 1, Palmitoyl-protein hydrolase 1, INCL, EC:3.1.2.22, CLN1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, pig heart tissue, pig brain tissue, LNCap cells, HeLa cells, Jurkat cells, K-562 cells, pig cerebellum tissue, rabbit brain tissue, rabbit cerebellum tissue
Positive IF-P detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67699-1-Ig targets PPT1 in WB, IF-P, ELISA applications and shows reactivity with human, pig, rabbit samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, pig, rabbit
免疫原 PPT1 fusion protein Ag30550 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 palmitoyl-protein thioesterase 1
别名 Palmitoyl-protein thioesterase 1, Palmitoyl-protein hydrolase 1, INCL, EC:3.1.2.22, CLN1
计算分子量 34 kDa
观测分子量32-34 kda
GenBank蛋白编号BC008426
基因名称 PPT1
Gene ID (NCBI) 5538
RRIDAB_2882891
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP50897
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The defective gene behind the INCL(infantile neuronal ceroid lipofuscinoses) disease, CLN1, encodes for palmitoyl protein thioesterase 1 (PPT1). It consists of 306 amino acids, including a signal sequence of 26 amino acids and three N-linked glycosylation sites. This protein exists with a molecular mass of 32 kDa, 34 kDa,36 kDa, and 38 kDa. The enzyme is transported into lysosomes of non-neuronal cells by the mannose 6-phosphate receptor (M6PR) mediated pathway and a significant amount of native PPT1 resides in a complex rather than in a monomeric form(PMID:17565660).

实验方案

Product Specific Protocols
WB protocol for PPT1 antibody 67699-1-IgDownload protocol
IF protocol for PPT1 antibody 67699-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols