验证数据展示
经过测试的应用
Positive IHC detected in | mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | rat pancreas tissue |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 1 publications below |
IF | See 2 publications below |
产品信息
15493-1-AP targets Pancreatic Polypeptide in IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | IHC, IF-P, FC (Intra), ELISA Application Description |
文献引用应用 | IHC, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | mouse |
免疫原 | Pancreatic Polypeptide fusion protein Ag7886 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | pancreatic polypeptide |
别名 | PPY, PH, Pancreatic prohormone, Pancreatic polypeptide Y, Pancreatic polypeptide prohormone |
计算分子量 | 10 kDa |
GenBank蛋白编号 | BC032225 |
基因名称 | Pancreatic Polypeptide |
Gene ID (NCBI) | 5539 |
RRID | AB_2878145 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P01298 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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IHC protocol for Pancreatic Polypeptide antibody 15493-1-AP | Download protocol |
IF protocol for Pancreatic Polypeptide antibody 15493-1-AP | Download protocol |
FC protocol for Pancreatic Polypeptide antibody 15493-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Transpl Int Formation of Re-Aggregated Neonatal Porcine Islet Clusters Improves In Vitro Function and Transplantation Outcome | ||
Vet Med Sci Histology and immunofluorescent study of the pancreas in lovebird (Agapornis personatus) | ||
Diabetes Mettl3-Mediated m6A Methylation Controls Pancreatic Bipotent Progenitor Fate and Islet Formation | ||