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PRAME Polyclonal antibody

PRAME Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11438-1-AP

Print datasheet

Synonyms

MAPE, OIP 4, OIP4, Opa interacting protein 4, PRAME

验证数据展示

  • WB analysis using 11438-1-AP

    WB analysis using 11438-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11438-1-AP (PRAME antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 11438-1-AP

    IP experiment of K-562 using 11438-1-AP

    IP result of anti-PRAME (IP:11438-1-AP, 4ug; Detection:11438-1-AP 1:300) with K-562 cells lysate 2400 ug.

  • IHC staining of human lung cancer using 11438-1-AP

    IHC staining of human lung cancer using 11438-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11438-1-AP (PRAME antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inK-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11438-1-AP targets PRAME in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human
Immunogen PRAME fusion protein Ag1906 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name preferentially expressed antigen in melanoma
Synonyms MAPE, OIP 4, OIP4, Opa interacting protein 4, PRAME
Calculated Molecular Weight 509 aa, 58 kDa
Observed Molecular Weight 50-58 kDa
GenBank Accession NumberBC014074
Gene Symbol PRAME
Gene ID (NCBI) 23532
RRIDAB_2169351
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP78395
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The PRAME (preferentially expressed antigen of melanoma) gene was previously shown to be overexpressed in ovarian/primary peritoneal serous carcinoma compared with malignant mesothelioma using gene expression arrays. It is considered a melanocyte differentiation antigen which is overexpressed in both solid and hematologic tumors. In normal tissue, a very low level of PRAME expression is found in normal testis, adrenals, ovary and endometrium. A high level of PRAME expression has been reported for several solid tumors, including ovarian cancer, breast cancer, lung cancer and melanomas, medulloblastoma, sarcomas, head and neck cancers, neuroblastoma, renal cancer, and Wilms'tumor. As a nuclear transcriptional repressor protein, PRAME binds to retinoic acid receptor a, thereby inhibiting retinoic acid induced differentiation, growth arrest, and apoptosis.

实验方案

Product Specific Protocols
WB protocol for PRAME antibody 11438-1-APDownload protocol
IHC protocol for PRAME antibody 11438-1-APDownload protocol
IP protocol for PRAME antibody 11438-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols